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KMID : 0614020090240010011
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Journal of Pharmaceutical Sciences (C.N.U.)
2009 Volume.24 No. 1 p.11 ~ p.23
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Nonviral Gene Delivery
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Park Joon-Won
Myung Chang-Seon
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Abstract
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The specific aim of gene therapy is to deliver a therapeutic target gene to cells in where the expression of transgene can be properly increased. Two main types to deliver a transgene are using viral vectors or nonviral approaches. Gene delivery using viral vectors shows long-term gene expression with high efficiency. However, since the issues about safety and the limitation of transgene size in recombinant viruses, nonviral approaches have been practically challenged. Nonviral approches can be divided two approaches; physical and chemical ones. In this review, the most commonly used nonviral methods were discussed with its mechanism of action for gene delivery. Moreover, the technical aspects of each delivery system were reviewed with their advantages and limitations for practical applications. Although the progress in vector design and the understanding of transfection biology has been remarkably made, continuous effort to improve currently available systems is needed for safer and more efficient nonvial gene delivery.
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KEYWORD
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nonviral gene delivery, gene therapy
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